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ORIGINAL ARTICLE
Year : 2016  |  Volume : 10  |  Issue : 1  |  Page : 9-14

Utility of quantitative real time PCR in detection and monitoring of viral infections in post renal transplant recipients


1 Department of Renal Transplant Surgery, Postgraduate Institute of Medical Education and Research, PGIMER, Chandigarh 160012, India
2 Department of Immunopathology, Postgraduate Institute of Medical Education and Research, PGIMER, Chandigarh 160012, India
3 Department of Nephrology, Postgraduate Institute of Medical Education and Research, PGIMER, Chandigarh 160012, India
4 Department of Histopathology, Postgraduate Institute of Medical Education and Research, PGIMER, Chandigarh 160012, India

Correspondence Address:
Yashwant Kumar
Department of Immunopathology, Postgraduate Institute of Medical Education and Research, PGIMER, Chandigarh 160012
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.1016/j.ijt.2016.03.007

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Background: Viral infections cause significant morbidity and mortality in post-transplant period. A highly sensitive and specific detection tool if used may help in early diagnosis and better management in these patients. The study aimed to assess the utility of quantitative real-time polymerase chain reaction (qRT-PCR) as a diagnostic and monitoring tool for viral infections in post renal transplant patients. Methods: A quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect EBV and CMV infection in 50 patients on 1st, 2nd, 3rd, and after 6 months of renal transplantation. Results: CMV infection was found in 34%, EBV in 28% of recipients, and 17% showed dual infection. Viruses were detectable after the first month of transplantation followed by symptomatic infections within first three months of follow-up, with diarrhea being the commonest symptom. These patients were also at high risk for developing other infections. Anti-thymocyte globulin (ATG) induction was a definitive risk factor for CMV/EBV infection in post operative period. Conclusion: Renal transplant patients frequently develop one or more viral infections at a time. Regular monitoring with qRT-PCR and prompt antiviral therapy with reduction in immunosuppression may be an ideal approach for management of these patients.


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