Indian Journal of Transplantation

ORIGINAL ARTICLE
Year
: 2020  |  Volume : 14  |  Issue : 4  |  Page : 293--297

Comparison of the CMV antigenemia and CMV-DNA QPCR results in haematopoetic stem cells transplanted recipients - A retrospective observational study


Habib Ksouri, Yosra Chebbi, Anis Raddaoui, Wafa Achour 
 Laboratory, National Bone Marrow Transplant Center, Tunis, Tunisia

Correspondence Address:
Dr. Habib Ksouri
Laboratory, National Bone Marrow Transplant Center: 2, Rue Jebel Lakhdar, Tunis 1006
Tunisia

Objectives: Diagnosis of active cytomegalovirus (CMV) infection in hematopoietic stem cell (HSC)-transplanted patients is essential because of the serious complications it causes, especially CMV disease. Such diagnosis allows the initiation of a preemptive antiviral therapy, which avoids the development of such disease. In this retrospective study made as a part of routine work for transplanted patients, we compared two diagnosis testing for CMV-active infection such as pp65 CMV antigenemia and a quantitative real-time polymerase chain reaction (QPCR). Our purpose was to assess the contribution of these tools to the diagnosis and monitoring of active CMV infections. Materials and Methods: Five hundred and fifty-eight and 156 samples belonging to 24 HSC transplanted patients were, respectively, tested by antigenemia and QPCR. Results: For the 156 samples analyzed by both testing, a number of positive samples by a QPCR were higher than that by antigenemia (44.8% vs. 13%), with a statistically significant difference (P < 0.0001). A statistically significant correlation between viral loads of these two testing was also found (rs = 0.765). In median, QPCR becomes positive 14 days before antigenemia (P < 0.0001) and its positivity lasts for 91 days, whereas antigenemia positivity lasts only for 56 days (P < 0.001). No statistically significant difference was found between the results of these two testing in CMV disease cases. Conclusions: QPCR is a rapid, standardized assay, which permits a precocious detection of CMV DNA. Kinetic of DNA evolution is a reliable diagnostic tool and more effective than antigenemia-based assays in monitoring CMV infection.


How to cite this article:
Ksouri H, Chebbi Y, Raddaoui A, Achour W. Comparison of the CMV antigenemia and CMV-DNA QPCR results in haematopoetic stem cells transplanted recipients - A retrospective observational study.Indian J Transplant 2020;14:293-297


How to cite this URL:
Ksouri H, Chebbi Y, Raddaoui A, Achour W. Comparison of the CMV antigenemia and CMV-DNA QPCR results in haematopoetic stem cells transplanted recipients - A retrospective observational study. Indian J Transplant [serial online] 2020 [cited 2021 Mar 7 ];14:293-297
Available from: https://www.ijtonline.in/article.asp?issn=2212-0017;year=2020;volume=14;issue=4;spage=293;epage=297;aulast=Ksouri;type=0